These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The immature B-cell subpopulation with low RAG1 expression is increased in the autoimmune New Zealand Black mouse.
    Author: Toda T, Kitabatake M, Igarashi H, Sakaguchi N.
    Journal: Eur J Immunol; 2009 Feb; 39(2):600-11. PubMed ID: 19180472.
    Abstract:
    Receptor editing is believed to play a critical role in immunological tolerance by altering the specificity of autoreactive B cells that emerge in the bone marrow. To study whether receptor editing is altered in autoimmune disease, recombination activation gene 1 (rag1) transcription in B cells from New Zealand Black (NZB) mice was examined by introducing a GFP gene at the rag1 locus. Female NZB(RAG1-GFP) mice generated autoantibodies and glomerular immune complexes. NZB(RAG1-GFP) mice did not display increased RAG1-GFP signal in B-1 and B-2 cells from the spleen and peritoneal cavity even following in vitro stimulation compared with C57BL/6(RAG1-GFP) control mice. The early B cells in the bone marrow were classified into RAG1-GFP(-) and RAG1-GFP(+) subpopulations. The RAG1-GFP(-) immature B-cell population was in the minority in C57BL/6(RAG1-GFP) mice but was markedly increased in NZB(RAG1-GFP) mice. RAG1-GFP(-) immature B cells from NZB(RAG1-GFP) mice differentiated into anti-dsDNA Ab-producing cells after stimulation by LPS in vitro. RAG1-GFP(-) immature B cells from NZB(RAG1-GFP) mice displayed a different expression profile of transcription factors required for receptor editing, including pax5 and irf4, in comparison with the RAG1-GFP(+) immature B-cell population. In conclusion, the early B-cell subpopulation, which exhibits low RAG1 expression and presumably concomitantly low receptor editing, was found to be increased in NZB mice.
    [Abstract] [Full Text] [Related] [New Search]