These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [MR R2*map for tracing superparamgnetic ironoxides labeled endothelial progenitor cells in vitro].
    Author: Wang QG, Yan FH, Xu PJ.
    Journal: Zhonghua Gan Zang Bing Za Zhi; 2009 Jan; 17(1):50-2. PubMed ID: 19203453.
    Abstract:
    OBJECTIVES: To investigate the value of R2*map for quantitatively tracing superparamgnetic ironoxides (SPIO) labeled endothelial progenitor cells (EPCs). METHODS: The EPCs were isolated from Balb/c mice bone marrow and cultured in vitro. After 7 days, expression of acetylated low-density lipoprotein (acLDL) and Ulex europaeus agglutinin-1 (UEA-1), two markers of EPCs, was observed by double staining using fluorescence microscope, the expression of stem cell antigen-1 and vascular endothelial growth factor receptor-2 (VEGFR-2) was confirmed by flow cytometry. EPCs were labeled by incubating with 50 microg/ml SPIO and 6 microl/ml lipofectamine2000, SPIO labeled EPCs were observed under transmission electron microscope (TEM). Labeled and unlabeled EPCs were mixed with 10 g/L agarose and scanned using a 3.0T MR scanner, R2* map and R2 map images were obtained on workstation. RESULT: After 7 days of in vitro culture, most of the cells showed characteristics of EPCs. There was no morphological difference between SPIO labeled EPCs and unlabeled EPCs. R2* and R2 values exhibited a linear correlation with the number of labeled cells in the agarose gel. Compared to R2, R2* was a better indicator of the number of labeled cells. CONCLUSION: MR R2* map can be used to trace SPIO labeled EPCs quantitatively.
    [Abstract] [Full Text] [Related] [New Search]