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  • Title: Isolation of functional polymorphonuclear leukocytes from rhesus monkeys using discontinuous Ficoll-Hypaque density gradients.
    Author: Taniuchi S.
    Journal: J Med Primatol; 1991 Jul; 20(5):271-4. PubMed ID: 1920384.
    Abstract:
    A simple, rapid method for separating polymorphonuclear leukocytes (PMNs) from adult rhesus monkey blood, based on the use of a discontinuous gradient of Ficoll-Hypaque (densities, 1.100, 1.077) has been developed. Using this method, 71.1% of the PMNS were recovered in a layer with a purity of 91% PMNs with very few contaminating erythrocytes. The added advantage of the method described here is that a higher chemotactic activity was retained.
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