These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Photobleaching absorbed Rhodamine B to improve temperature measurements in PDMS microchannels.
    Author: Glawdel T, Almutairi Z, Wang S, Ren C.
    Journal: Lab Chip; 2009 Jan 07; 9(1):171-4. PubMed ID: 19209352.
    Abstract:
    Rhodamine B based fluorescence thermometry is commonly used in microfluidics to measure fluid temperatures in microchannels. Notable absorption of Rhodamine B into PDMS channel walls, however, causes difficulties in obtaining accurate temperature measurements due to a steady increase in the overall fluorescent signal. A simple and effective technique is reported that removes the fluorescent signal from absorbed Rhodamine B dye by means of photobleaching with a high intensity light source before taking images for thermometry analysis. The temperature field at the convergence of hot and cold streams in a Y-channel fabricated in PDMS/glass microfluidic chip is studied to demonstrate the execution of the photobleaching technique.
    [Abstract] [Full Text] [Related] [New Search]