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  • Title: Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta.
    Author: Haibo Z, Yinglong Z, Feng H, Peiji G, Jiachuan C.
    Journal: Biotechnol Lett; 2009 Jun; 31(6):837-43. PubMed ID: 19221878.
    Abstract:
    A laccase was purified from Trametes hirsuta. This laccase was classified as a "white" or "yellow" laccase. pH 2.4 was optimal for the oxidation of ABTS and pH 2.5 for DMP. DMP oxidation was optimal at 85 degrees C. The half-life of this laccase was 70 min at 75 degrees C, and 5 h at 65 degrees C. Non-phenolic dyes, such as Methyl Red, were oxidized by purified laccase without mediators. The enzyme was not inhibited by Cu(2+), Mn(2+), or EDTA. These are atypical laccase characteristics that make it a good candidate for theoretical and applied research.
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