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  • Title: A DNA biosensor based on the electrocatalytic oxidation of amine by a threading intercalator.
    Author: Gao Z, Tansil N.
    Journal: Anal Chim Acta; 2009 Mar 16; 636(1):77-82. PubMed ID: 19231359.
    Abstract:
    An electrochemical biosensor for the detection of DNA based a peptide nucleic acid (PNA) capture probe (CP) modified indium tin oxide electrode (ITO) is described in this report. After hybridization, a threading intercalator, N,N'-bis[(3-propyl)-imidazole]-1,4,5,8-naphthalene diimide (PIND) imidazole complexed with Ru(bpy)(2)Cl (PIND-Ru, bpy=2,2'-bipyridine), was introduced to the biosensor. PIND-Ru selectively intercalated to double-stranded DNA (ds-DNA) and became immobilized on the biosensor surface. Voltammetric tests showed highly stable and reversible electrochemical oxidation/reduction processes and the peak currents can directly be utilized for DNA quantification. When the tests were conducted in an amine-containing medium, Tris-HCl buffer for example, a remarkable improvement in the voltammetric response and noticeable enhancements of voltammetric and amperometric sensitivities were observed due to the electrocatalytic activity of the [Ru(bpy)(2)Cl] redox moieties. Electrocatalytic current was observed when as little as 3.0attomoles of DNA was present in the sample solution.
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