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  • Title: Construction and tropism characterisation of recombinant viruses exhibiting HIV-1 env gene from seminal strains.
    Author: Lawrence P, Berlier W, Delezay O, Palle S, Olivier T, Saoudin H, Mottin S, Lucht F, Pozzetto B, Bourlet T.
    Journal: Virology; 2009 Apr 10; 386(2):373-9. PubMed ID: 19232661.
    Abstract:
    Genetic differences between blood and mucosal-derived HIV-1 strains have been widely reported. As amplification of HIV-1 strains from mucosal samples including semen or saliva by co-culture has low sensitivity, we developed the construction of chimeric viruses expressing wild-type seminal HIV-1 envelope protein. Chimeric viruses were produced by co-transfection of a V1-V3 deleted pNL 43 vector and PCR fragments spanning the deleted region, amplified from HIV-1 RNA positive seminal plasma samples. After an initial testing of co-receptor usage by a tropism recombinant test, replication capacity and amplification of these recombinant viruses were assessed using PBMC. Four chimeric replicative strains, all using CXCR4 as coreceptor, were produced. The interaction between cell-free viral particles and reporter cell lines was assessed by confocal microscopy. These replicative chimeras exhibiting HIV-1 env from seminal strains represent useful tools for the in vitro study of the heterosexual transmission of HIV-1 and testing of microbicide activity.
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