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  • Title: [On hexokinase isozymes].
    Author: Gobeev VN, Khripach LV.
    Journal: Biokhimiia; 1976 Aug; 41(8):1504-9. PubMed ID: 192340.
    Abstract:
    Electrophoretic study of hexokinase (HK) associated with the soluble fraction of mouse transplantable hepatoma 22a revealed that almost all bands of HK activities overlapped the bands of glucose-6-P dehydrogenase (G6PDH) activities in the gels. Similar results were obtained for liver, muscle and brain soluble fractions, as well as for various extracts from hepatoma 22a mitochondria and commercial preparation of yeast HK. A single type of HK, which does not overlap G6PDH activity, was located between types I and II (according to the Katzen classification) as a diffuse band of 1 hour manifestation. A possibility of structural organization of glycolytic enzymes in the cell essential for the quantitative estimation of the isozyme pattern is discussed.
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