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  • Title: [Interaction of thiamine pyrophosphokinase from brewer's yeast with thiamine and adenosine-5'-triphosphate].
    Author: Voskoboev AI, Chernikevich IP.
    Journal: Biokhimiia; 1977 Jan; 42(1):79-84. PubMed ID: 192344.
    Abstract:
    The interaction of thiamine pyrophosphokinase (Thiaminkinase EC 2.7.6.2) with thiamine and ATP was studied. It was shown that the mechanism of the thiaminokinase reactions is Rapid Equilibrium Random Bi -- Bi. The enzyme binds 8 moles ATP and 1 mole thiamine per mole protein Ks = 0,8-10(-3) and 4.10(-6) M for ATP and thiamine respectively.
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