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  • Title: [Effects of Bufalin on SYK and CBL family proteins in induction of HL-60 cell apoptosis].
    Author: Qu XJ, Zhao MF, Liu YP, Teng YE, Qu JL, Zhang Y, Xu L, Li YC, Hou KZ.
    Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi; 2009 Feb; 17(1):65-8. PubMed ID: 19236749.
    Abstract:
    The study was aimed to explore the mechanism of SYK and CBL family of ubiquitin ligases in Bufalin-induced HL-60 cells apoptosis. Cell viability was tested by trypan blue staining and apoptosis was detected by using flow cytometry. The expressions of CBL and CBL-b and the phosphorylation of SYK were detected by using immunoprecipitation and Western blot. The results showed that Bufalin inhibited HL-60 cell proliferation in time- and dose-dependent manners. IC(50) of suppressing cell viability at 24, 48 and 72 hours were about 26.3, 7.8 and 2.0 nmol/L respectively. The high dose of bufalin already induced apoptosis of HL-60 cells at 8 hours. SYK was quickly phosphorylated, and the expressions of CBL and CBL-b were down-regulated after treatment with Bufalin. It is concluded that SYK activation and CBL protein down-regulation may be involved in Bufalin-induced HL-60 cell apoptosis.
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