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  • Title: Effect of progesterone on HLA-E gene expression in JEG-3 choriocarcinoma cell line.
    Author: Huang Z, Hyodo H, Fujii T, Nagamatsu T, Matsumoto J, Kawana K, Yamashita T, Yasugi T, Kozuma S, Taketani Y.
    Journal: Am J Reprod Immunol; 2009 Mar; 61(3):221-6. PubMed ID: 19239424.
    Abstract:
    PROBLEM: Among class Ib human leukocyte antigen (HLA) molecules, HLA-E is known to be a major ligand of CD94/NKG2 receptor on natural killer (NK) cells, and to play a pivotal role in recognition of extravillous trophoblasts (EVTs) by maternal immune cells. However, it is scarcely known how HLA-E expression is regulated in EVTs. METHOD OF STUDY: In this study, we investigated whether progesterone, an essential hormone in maintaining pregnancy, regulated HLA-E expression in EVT-like cell line, JEG-3. HLA-E mRNA amount in cultured JEG-3 cells was assessed by real-time PCR and cell-surface HLA-E protein was analyzed by flowcytometry. RESULTS: Real-time PCR showed 3.5-fold increase 1 hour after the addition of 1000 ng/ml progesterone. This response was diminished by the addition of RU486, an antagonist for progesterone receptor. Flowcytometry indicated that 1000 ng/ml progesterone slightly enhanced HLA-E expression on the surface of JEG-3. CONCLUSION: These results suggest that progesterone up-regulates HLA-E expression in JEG-3 cells through the pathway mediated by progesterone receptor. Our findings might give a new insight into immunomodulatory function of progesterone at fetomaternal interface.
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