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  • Title: Construction and expression of a novel bioactive IFN-alpha2b/CM4 fusion protein in Escherichia coli.
    Author: Li NN, Liu P, Chen SJ, Lin QP, Zhou LF, Zhang SQ.
    Journal: Microbiol Res; 2010 Feb 28; 165(2):116-21. PubMed ID: 19246180.
    Abstract:
    Human interferon alpha2b (IFN-alpha2b) is a pleiotropic cytokine used for the treatment of various cancers. Antibacterial peptide CM4 is a small peptide that can strongly inhibit many kinds of bacteria, fungi, and tumor cells, but it does no harm to normal cells. Here, we describe a protein expression system for the production of IFN-alpha2b/CM4 fusion protein in insoluble form in Escherichia coli, coupled to an efficient dialysis refolding and histidine-tag purification protocol. The expressed IFN-alpha2b/CM4 fusion protein not only displays significantly improved antitumor activity, but also retains the antibacterial-antifungal activity of CM4.
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