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  • Title: Molecular characterization of an atoxigenic Aspergillus flavus strain AF051.
    Author: Jiang J, Yan L, Ma Z.
    Journal: Appl Microbiol Biotechnol; 2009 Jun; 83(3):501-5. PubMed ID: 19255755.
    Abstract:
    An atoxigenic Aspergillus flavus strain AF051 collected from a peanut field in Jiangsu province, P. R. China was characterized by analysis of aflatoxin gene cluster in this study. By using a thermal asymmetric interlaced PCR (TAIL-PCR) and conventional PCR techniques, an 89.59-kb deletion was found in the cluster, and this deletion was replaced by a 3.83-kb insert, which was located at 300-bp upstream ver1 gene and 2594-bp downstream a putative gluconolactone oxidase gene. Based on the DNA sequence at the breakpoint, a nested-PCR method was developed for the rapid and sensitive detection of AF051 strain in soil and peanut samples once the strain is used as a biological agent.
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