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Title: [Application of flow microbeads assay in detection of specific antibodies and cytokines in serum from patients with hemorrhagic fever with renal syndrome].
Author: Zhang J, Lin Y, Jin BQ.
Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2009 Mar; 25(3):245-7. PubMed ID: 19257990.
Abstract:
AIM: To establish a flow microbeads assay (FMA) to examine the level of hemorrhagic fever with renal syndrome virus (HFRS V.) specific IgM, IgG and cytokines in HFRS patients. METHODS: Serum samples from 28 cases of HFRS and 20 healthy controls were studied. Serum levels of anti-HFRS V. antibodies were qualified and inflammatory cytokines IL-6 and TNF-alpha were quantified by FMA. The results were compared with the results by ELISA. RESULTS: FMA showed that the positivity rates for anti-HFRS V. IgM and IgG were 92.85% and 71.43%, respectively. None was detected positive in healthy control group. The serum level of IL-6 and TNF-alpha in HFRS group were (532.62+/-397.19) ng/L and (392.68+/-177.68)ng/L, respectively, which were significantly higher than that in healthy control group (38.77+/-20.32 ng/L and 15.91+/-6.91 ng/L, P<0.01). In contrast, ELISA showed that the positivity rates for anti-HFRS V. IgM and IgG were 71.43% and 50.00%, respectively. None was detected positive in healthy control group. The serum level of IL-6 and TNF-alpha in HFRS group were (256.46+/-102.51) ng/L ang (45.63+/-5.32) ng/L, respectively, which were significantly higher than that in healthy control group (53.80+/-19.21 ng/L and 5.81+/-3.58 ng/L, P<0.01). CONCLUSION: A FMA method is established to monitor the immune response in HFRS patients, which has better sensitivity than ELISA, and thus provides a useful tool for study of HFRS.

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