These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [The effect of PKC activation and Smac release on inhibition of myocardial cell apoptosis by Sini Decoction].
    Author: Liu Y, Nie YM, Wu WK.
    Journal: Zhong Yao Cai; 2008 Nov; 31(11):1675-8. PubMed ID: 19260278.
    Abstract:
    OBJECTIVE: To investigate the effect of Sini Decoction on myocardial cell apoptosis after MI/R and the possible relation to myocardial cell apoptosis, PKC and Smac. METHODS: Sprage-Dawley rats were divided into four groups: control group, sham group, MI/R group and SND group. The rats in MI/R group, left anterior descending coronary artery (LAD) was occluded for 1 h and reperfused for 1 h. The rats in SND group were pretreated with Sini decoction(5 g/kg x d) for three days, the last treatment was pretreated 24 h before the index occlusion. Cell apoptosis was measured by flow cytometry, the expression of PKC was measured by immunohistochemistry. The expression of Smac was analyzed by Western blotting. The activity of Caspase-3 was also measured. RESULTS: 24 h after Sini Decoction treatment, Myocardial cell apoptosis significantly decreased, PKC was translocated and its expression increased, the release of Smac decreased, the activity of Caspase-3 decreased. CONCLUSION: Sini Decoction treatment can protect myocardial cell from apoptosis caused by MI/R, the mechanism is related to PKC activation, Mitochondria protection and in turn inhibition of Smac release, and inhibition of apoptosis signal transduction.
    [Abstract] [Full Text] [Related] [New Search]