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  • Title: [Isolation-preparation and determination of chromones from Saposhnikovia divaricata].
    Author: Zhang C, Xiao YQ, Li L, Pang Z, Li GL.
    Journal: Zhongguo Zhong Yao Za Zhi; 2008 Dec; 33(23):2761-4. PubMed ID: 19260305.
    Abstract:
    OBJECTIVE: To compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata. METHOD: The separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1). RESULT: All the obtained correlation coefficient of the six components was over 0.9995 and the average recoveries were not less than 97% (RSD <5%), respectively. CONCLUSION: The method is simple, accurate with good reproducibility. Results showed the better preparation procedure is water-decoction and ethanol-precipitation with resin separation and silica gel purification.
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