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  • Title: [Determination of anthraquinone in Semen Cassiae from different regions by HPLC].
    Author: Zhang Y, Huang XP, Weng DQ, Yang DJ.
    Journal: Zhongguo Zhong Yao Za Zhi; 2008 Dec; 33(23):2797-9. PubMed ID: 19260315.
    Abstract:
    OBJECTIVE: To determine the content of 7 anthraquinones in Semen Cassiae. METHOD: A HPLC method was developed, with Inertsil ODS-3 column, acetonitrile and 0.1% H3PO4 solution as mobile phases in gradient elution. The detection wavelength wasset at 278 nm, and the flow rate was 0.8 mL x min(-1). RESULT: Recoveries of all 7 anthraquinones were between 95%-105%. The content of the anthraquinones in crude drug produced in different habitation were different. CONCLUSION: The method is convenient and accurate, which provides the foundation for the research of Semen Cassiae.
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