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  • Title: First isolation and characterization of canine distemper virus in Vietnam with the immunohistochemical examination of the dog.
    Author: Lan NT, Yamaguchi R, Kien TT, Hirai T, Hidaka Y, Nam NH.
    Journal: J Vet Med Sci; 2009 Feb; 71(2):155-62. PubMed ID: 19262025.
    Abstract:
    Canine distemper caused by canine distemper virus (CDV) is a contagious, incurable, often fatal, multisystemic viral disease that affects the respiratory gastrointestinal and central nervous system. Strains Vn86 and Vn99 of CDV were isolated, we believe for the first time, in Vietnam from two 4-month-old autopsied dogs pathologically showing non-suppurative encephalitis with pneumonia, lymphoid depletion and severe gastroenteritis. These strains caused syncytium cytopathic effect in Vero cells and Vero cells expressing canine signaling lymphocyte activation molecules. The titers of cell-associated viruses of both strains were higher than for released viruses. Molecular analysis showed that both new isolates of CDV joined to the group of classic type that is far from the Asia 1 and Asia 2 groups. These results indicated that first isolation and characterization of canine distemper virus in Vietnam with the immunohistochemical examination of the dog.
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