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  • Title: Rapid detection of Listeria monocytogenes in food by polymerase chain reaction.
    Author: Ennaji H, Timinouni M, Ennaji MM, Ait m'hand R, Hassar M, Cohen N.
    Journal: Cell Mol Biol (Noisy-le-grand); 2009 Feb 25; 55 Suppl():OL1104-10. PubMed ID: 19267993.
    Abstract:
    The standard conventional methods for the detection of Listeria monocytogenes in foods require high time 7 to 10 days to give ready results. To dissolve this problem we have evaluate a short method using Polymerase Chain Reaction (PCR) to analyze food samples. In parallel with this study, a comparison was made between PCR amplification from templates directly prepared from food and the official standard ISO procedure 11290-1. In this study we have used a Half Frazer broth as an enrichment medium; there were positive results of PCR detection of L. monocytogenes in different food sample analyzed (milk, cheese and meat) with approximately 1.5 10(1) Colony Forming Units /25 g in less than 36 h. This PCR procedure has proved to be rapid and sensitive method suitable for the routine analysis; firstly, because this assay required just a short pre-enrichment step before PCR. Secondly, this procedure is very simple and time-saving; it could take less than one working day to obtain results if initial microbiological load was very important.
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