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  • Title: Development of an ICP-MS immunoassay for the detection of anti-erythropoietin antibodies.
    Author: Lu Y, Wang W, Xing Z, Wang S, Cao P, Zhang S, Zhang X.
    Journal: Talanta; 2009 May 15; 78(3):869-73. PubMed ID: 19269443.
    Abstract:
    A sandwich-type immunoassay linked with inductively coupled plasma mass spectrometry (ICP-MS) has been developed for the detection of anti-erythropoietin antibodies (anti-EPO Abs). Recombinant human erythropoietin (rhEPO) was immobilized on the solid phase to capture anti-rhEPO Abs specifically. After the immunoreactions with Au-labeled goat-anti-rabbit IgG, a diluted HNO(3) (2%) was used to dissociate Au nanoparticles which was then introduced to the ICP-MS for measurements. Under the optimized conditions, the calibration graph for anti-EPO Abs was linear in the range of 35.6-500 ng mL(-1) with a detection limit of 10.7 ng mL(-1) (3sigma, n=9). The relative standard deviation (R.S.D.) for three replicate measurements of 30.9 ng mL(-1) of anti-EPO Abs was 8.43%. The recoveries of anti-EPO Abs in sera at the spiking level of 50, 100, 150, 200 and 400 ng mL(-1) were 99.2%, 101.5%, 95.0%, 94.0% and 102.9%, respectively. For the real sample analysis, 26 samples from healthy people and 53 samples from patients with rhEPO treatments were studied. One sample from patients showed significantly higher anti-EPO Abs from other samples, indicating a possibility of immune response of this patient.
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