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Title: High-throughput and high-sensitivity nano-LC/MS and MS/MS for O-glycan profiling. Author: Karlsson H, Larsson JM, Thomsson KA, Härd I, Bäckström M, Hansson GC. Journal: Methods Mol Biol; 2009; 534():117-31. PubMed ID: 19277531. Abstract: Sensitive and fast methods for the profiling of biologically important molecules are highly demanded. Mucins are densely O-glycosylated glycoproteins found at mucosal surfaces and are of great medical interest. Here we describe sensitive methods for the analysis of O-glycans from mucins using gel electrophoresis, and chromatography by nanoLC on graphite columns and structural analysis by electrospray mass spectrometry on a linear trap mass spectrometer.[Abstract] [Full Text] [Related] [New Search]