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  • Title: A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat.
    Author: van den Broeck HC, America AH, Smulders MJ, Bosch D, Hamer RJ, Gilissen LJ, van der Meer IM.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2009 Apr 01; 877(10):975-82. PubMed ID: 19282254.
    Abstract:
    The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, currently different extraction protocols exist. The performance of various documented gluten extraction protocols is evaluated for specificity and completeness by gel electrophoresis (SDS-PAGE), immunoblotting and RIDASCREEN Gliadin competitive ELISA. Based on these results, an optimized, two-step extraction protocol has been developed.
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