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  • Title: Visualization of plastids in pollen grains: involvement of FtsZ1 in pollen plastid division.
    Author: Tang LY, Nagata N, Matsushima R, Chen Y, Yoshioka Y, Sakamoto W.
    Journal: Plant Cell Physiol; 2009 Apr; 50(4):904-8. PubMed ID: 19282372.
    Abstract:
    Visualizing organelles in living cells is a powerful method to analyze their intrinsic mechanisms. Easy observation of chlorophyll facilitates the study of the underlying mechanisms in chloroplasts, but not in other plastid types. Here, we constructed a transgenic plant enabling visualization of plastids in pollen grains. Combination of a plastid-targeted fluorescent protein with a pollen-specific promoter allowed us to observe the precise number, size and morphology of plastids in pollen grains of the wild type and the ftsZ1 mutant, whose responsible gene plays a central role in chloroplast division. The transgenic material presented in this work is useful for studying the division mechanism of pollen plastids.
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