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  • Title: Rapid and label-free bacteria detection by surface plasmon resonance (SPR) biosensors.
    Author: Dudak FC, Boyaci IH.
    Journal: Biotechnol J; 2009 Jul; 4(7):1003-11. PubMed ID: 19288516.
    Abstract:
    Surface Plasmon Resonance (SPR) biosensor technology has been successfully used for the detection of various analytes such as proteins, drugs, DNA, and microorganisms. SPR-based immunosensors that coupled with a specific antigen-antibody reaction, have become a promising tool for the quantification of bacteria as it offers sensitive, specific, rapid, and label-free detection. In this paper, we review the important issues in the development of SPR-based immunoassays for bacteria detection, concentrating on instrumentation, surface functionalization, liquid handling, and surface regeneration. In addition, this review touches on the recent advances in SPR biosensing for sensitivity enhancement.
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