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  • Title: 7beta-hydroxycholesterol induces apoptosis and regulates cyclooxygenase 2 in head and neck squamous cell carcinoma.
    Author: Heiduschka G, Erovic BM, Vormittag L, Skoda C, Martinek H, Brunner M, Ehrenberger K, Thurnher D.
    Journal: Arch Otolaryngol Head Neck Surg; 2009 Mar; 135(3):261-7. PubMed ID: 19289704.
    Abstract:
    OBJECTIVE: To determine whether treatment with 7beta-hydroxycholesterol (7beta-HC) would trigger cell death in head and neck squamous cell carcinoma (HNSCC) cell lines in a dose-dependent fashion. DESIGN: In vitro study. SUBJECTS: The study included HNSCC cell lines SCC9, SCC25, CAL27, and FaDu. INTERVENTION: We treated HNSCC cell lines with increasing doses of 7beta-HC. Proliferation assays were performed to assess cell viability after treatment. Western blots were carried out to evaluate cyclooxygenase (COX)-1 and -2 expression levels. RESULTS: Using proliferation assays and immunocytochemical analysis, we detected significant growth inhibition via apoptosis in 4 different HNSCC cell lines after treatment with 7beta-HC (P < .001). The 50% inhibitory concentration levels were between 13.19 and 20.79 micromol/L after 72 hours. Western analysis indicated that COX-2, but not COX-1, levels were suppressed after treatment. CONCLUSIONS: Treatment with 7beta-HC resulted in suppression of HNSCC growth in vitro. Our data warrant further investigations for the potential use of 7beta-HC as a cytotoxic agent in head and neck cancer.
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