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  • Title: Development of a method for the determination of vardenafil in human plasma by high performance liquid chromatography with UV detection.
    Author: Carlucci G, Palumbo P, Iuliani P, Palumbo G.
    Journal: Biomed Chromatogr; 2009 Jul; 23(7):759-63. PubMed ID: 19296519.
    Abstract:
    A simple high-performance liquid chromatographic (HPLC) method with photometric detection is described for the determination of vardenafil hydrochloride, a phosphodiesterase V inhibitor, in human plasma. Chromatographic separation of the analyte and internal standard was achieved on an analytical 250 x 4.6 mm i.d. reversed-phase Kromasil KR 100 C(18) (5 microm particle size) column using a mobile phase of acetonitrile-potassium dihydrogen phosphate (30:70 v/v). The run time was less than 15 min. Column eluate was monitored at 230 nm. The linearity over the concentration range of 10-1500 ng/mL for vardenafil was obtained and the limit of quantification (LOQ) was 10 ng/mL. The method has been applied to analysis of the vardenafil concentrations for application in pharmacokinetic studies.
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