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  • Title: Detection of myeloperoxidase activity in primary leukemic cells by an enhanced chemiluminescent assay for differentiation between acute lymphoblastic and non-lymphoblastic leukemia.
    Author: Tan S, Wang G, Peng M, Zhang X, Shen G, Jiang J, Chen F.
    Journal: Clin Chim Acta; 2009 May; 403(1-2):216-8. PubMed ID: 19298796.
    Abstract:
    BACKGROUND: Myeloperoxidase (MPO) plays a crucial role in the differentiation of acute lymphoblastic leukemia (ALL) and acute non-lymphoblastic leukemia (ANLL). In this report, we proposed the application of the enhanced chemiluminescent (ECL) technique to the determination of MPO activity in blasts of acute leukemia (AL). METHODS: Bone-marrow samples were obtained from 23 patients with AL (ALL, 5 cases; ANLL, 13 cases; AUL, 1 cases; mixed-lineage AL, 4 cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured. The mean peak light emission (PLE) was assessed. RESULTS: When the cut-off point of PLE was settled at 2483, which was set for the discrimination between ANLL and ALL (mean + 3 x SD of ALL samples, n=5), all cases of ALL were MPO-negative, and ten of the thirteen ANLL patients were MPO-positive, which was concordant with cytochemical staining. In addition, this technique was able to demonstrate MPO activity in 4 mixed-lineage AL cases which did not stain for MPO in cytochemistry preparations. CONCLUSIONS: Our ECL technique is simple, inexpensive, and easier to perform compared to other procedures used to measure MPO activity in AL.
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