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  • Title: Subcellular localization of acidic and basic PR proteins in tobacco mosaic virus-infected tobacco.
    Author: Dore I, Legrand M, Cornelissen BJ, Bol JF.
    Journal: Arch Virol; 1991; 120(1-2):97-107. PubMed ID: 1929881.
    Abstract:
    Infection of Samsum NN tobacco with tobacco mosaic virus (TMV) results in the induction of the synthesis of acidic and basic isoforms of many pathogenesis-related (PR) proteins. By immunogold-electromicroscopy we have shown that PR proteins accumulate mainly in cells around the necrotic spots of TMV-induced lesions. The acidic chitinases, beta-(1,3)-glucanases and thaumatin-like proteins were found to accumulate in extracellular "pocket-like" vesicles while the basic chitinases were found in electron dense inclusion bodies in the vacuoles. These structures were not detectable in PR-containing leaves devoid of virus nor in healthy plants.
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