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  • Title: Purification and characterization of a novel ribosome-inactivating protein from seeds of Trichosanthes kirilowii Maxim.
    Author: Shu SH, Xie GZ, Guo XL, Wang M.
    Journal: Protein Expr Purif; 2009 Oct; 67(2):120-5. PubMed ID: 19303444.
    Abstract:
    A novel ribosome-inactivating protein, designated Trichosanthrip, was purified from mature seeds of Trichosanthes kirilowii Maxim by cation-exchange and gel-filtration chromatography. Trichosanthrip migrated as a single band in SDS-PAGE, with an apparent molecular mass of approximately 13kDa. The molecular mass of Trichosanthrip was 10,964.617Da as determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Trichosanthrip showed N-glycosidase activity on 28 S rRNA and strongly inhibited cell-free protein synthesis, with an IC(50) of 1.6ng/ml. Liquid chromatography-tandem mass spectrometry showed that Trichosanthrip was a novel protein with similar sequence to other proteins present in members of the Cucurbitaceae.
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