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  • Title: [Detection and identification of mycobacterial gene in skin lesions and lymph nodes in patients with sarcoidosis].
    Author: Ding XL, Cai L, Zhang JZ.
    Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 2009 Feb; 31(1):20-3. PubMed ID: 19317051.
    Abstract:
    OBJECTIVE: To investigate the possible role of mycobacteria in the pathogenesis of sarcoidosis. METHODS: We used nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) and gene sequencing method to examine 31 formalin-fixed, paraffin-embedded specimens (including 19 skin lesions and 12 lymph nodes ) obtained from patients with sarcoidosis, 14 normal skin specimens, and 3 cutaneous tuberculosis specimens. RESULTS: The 65kD mycobacterial heat shock protein gene was found in 4 out of 19 (21.1%) skin specimens of sarcoidosis. The mycobacteria included M. tuberculosis (n = 1), M. chelonei (n = 2), and M. gordonae (n = 1). Mycobacterial DNA was negative in 12 lymph node specimens and 14 normal skin specimens. M. tuberculosis gene was detected in all 3 specimens of cutaneous tuberculosis. CONCLUSION: Mycobacteria may play a role in the pathogenesis of cutaneous sarcoidosis.
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