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  • Title: Crystallization and preliminary X-ray crystallographic analysis of SMU.412c protein from the caries pathogen Streptococcus mutans.
    Author: Ye ZY, Hou QM, Li LF, Su XD.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2009 Apr 01; 65(Pt 4):392-4. PubMed ID: 19342789.
    Abstract:
    The smu.412c gene encodes a putative histidine triad-like protein (SMU.412c) with 139 residues that is involved in cell-cycle regulation in Streptococcus mutans. The gene was cloned into the expression vector pET28a and subsequently expressed in Escherichia coli strain BL21 (DE3) to give a substantially soluble form of SMU.412c with a His(6) tag at its N-terminus. The recombinant protein was purified to homogeneity in a two-step procedure involving Ni(2+)-chelating and size-exclusion chromatography. Crystals suitable for X-ray diffraction were obtained using the sitting-drop vapour-diffusion method and diffracted to 1.8 A resolution on beamline BL6A at Photon Factory, Tsukuba, Japan. The crystal belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 53.5, c = 141.1 A.
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