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  • Title: Mass spectrometry-compatible silver staining of histones resolved on acetic acid-urea-Triton PAGE.
    Author: Pramod KS, Bharat K, Sanjay G.
    Journal: Proteomics; 2009 May; 9(9):2589-92. PubMed ID: 19343714.
    Abstract:
    Acetic acid-Urea-Triton (AUT) PAGE is commonly used method to separate histone variants and their post-translationally modified forms. Coomassie staining is the preferred method for protein visualization; however, its sensitivity is less than that of silver staining. Though silver staining of histones in AUT-PAGE has been reported, the method is time-consuming, dependent on prior staining by Amido black and has not been reported suitable for mass spectrometry. Here, we propose 'SDS-Silver' method for rapid, sensitive and mass spectrometry-compatible staining of histones resolved on AUT-PAGE.
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