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  • Title: Selective sensing of cysteine on manganese dioxide nanowires and chitosan modified glassy carbon electrodes.
    Author: Bai YH, Xu JJ, Chen HY.
    Journal: Biosens Bioelectron; 2009 Jun 15; 24(10):2985-90. PubMed ID: 19345085.
    Abstract:
    We report a simple approach for the selectively electrochemical sensing of cysteine (CySH) using beta-MnO(2) nanowires modified glassy carbon (GC) electrode via a direct and facile electrochemical co-deposition process with chitosan hydrogel. The electrode showed excellent electrocatalytic effect toward cysteine oxidation. A possible reaction mechanism related to the formation of surface complexes was proposed: the -SH group is the reacting group in the reaction between cysteine and MnO(2), while the -NH(2) group acts as an anchor to stabilize the surface complexes. The electrocatalytic behavior is further developed as a sensitive detection scheme for cysteine by amperometric measurement, which shows a large determination range of 0.5-630 microM and a low detection limit of 70 nM. Other nineteen amino acids in a 20-fold concentration do not interfere in the detection of cysteine. Furthermore, other biological species containing -SH group or S-S bonds, such as glutathione, cystine, and bovine serum albumin (BSA), show no interferences because of steric hindrance and inactive S-S bonds.
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