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  • Title: The number distribution of complex shear modulus of single cells measured by atomic force microscopy.
    Author: Hiratsuka S, Mizutani Y, Tsuchiya M, Kawahara K, Tokumoto H, Okajima T.
    Journal: Ultramicroscopy; 2009 Jul; 109(8):937-41. PubMed ID: 19345501.
    Abstract:
    The viscoelastic properties of a large number of mouse fibroblast NIH3T3 cells (n approximately 130) were investigated by combining atomic force microscopy (AFM) with a microarray technique. In the experiments, the cells were arranged and cultured in the wells of a microarray substrate, and a force modulation mode experiment was used to measure the complex shear modulus, G*, of individual cells in a frequency range 0.5-200Hz. The frequency dependence of G* of the cells exhibited a power-law behavior and similar frequency dependencies have been observed in several cell types cultured on flat substrates. This indicated that the NIH3T3 cells cultured in the wells of a microarray have analogous structural organization to those cells cultured on flat substrates. The number distribution of both the storage and loss moduli of G* fitted well to a log-normal distribution function, whereas the power-law exponent estimated by a power-law structural damping model showed a normal distribution function. These results showed that combining AFM with a microarray technique was a suitable approach for investigating the statistics of rheological properties of living cells without the requirement of cell surface modification.
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