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  • Title: Identification of trophoblast-specific binding sites for GATA-2 that are essential for rat placental lactogen-I gene expression.
    Author: Kim GS, Ko YG, Park OS, Park HJ, Koh PO, Cho KW, Min KS, Seong HH, Won CK, Cho JH.
    Journal: Biotechnol Lett; 2009 Aug; 31(8):1173-81. PubMed ID: 19357811.
    Abstract:
    We identified a 3.4-kb 5'-flanking region of the rPL-I gene and examined its promoter activity using rat trophoblast Rcho-1 cells. A regulatory element between base pairs (bp) -2,487 and -2,310 in the 5'-flanking region was essential for maximum promoter activity of the rPL-I gene. This regulatory element was further characterized between bp -2,443 to -2,415 and -2,374 to -2,345. Electrophoretic mobility shift analysis showed that the interaction of nuclear extract proteins from differentiated Rcho-1 cells was inhibited by competition with a GATA-like sequence in the promoter, but not by a mutated GATA sequence. Moreover, the promoter activity of 2487 eLuc containing two novel GATA sites was significantly elevated by co-transfection of a GATA-2 expression vector in proliferating Rcho-1 cells. Our results demonstrate that GATA-2 is involved in multiple promoter regions to activate the specific expression of the rPL-I gene in placental tissue.
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