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  • Title: A new strategy to improve a cauliflower mosaic virus vector.
    Author: Hirochika H, Hayashi K.
    Journal: Gene; 1991 Sep 15; 105(2):239-41. PubMed ID: 1937020.
    Abstract:
    Co-infection of plants with non-overlapping deletion mutants of cauliflower mosaic virus usually leads to the production of the wild-type virus. To prevent this, a pair of mutants with overlapping deletions was constructed. In infected plants both mutant DNAs were stably maintained. Such mutants with overlapping deletions will be used as a vector to overcome the size limitation of genes to be cloned.
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