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  • Title: HPLC with pulsed amperometric detection for sorbitol as a biomarker for diabetic neuropathy.
    Author: Sim HJ, Jeong JS, Kwon HJ, Kang TH, Park HM, Lee YM, Kim SY, Hong SP.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2009 May 15; 877(14-15):1607-11. PubMed ID: 19372065.
    Abstract:
    This study describes the optimal analytical conditions for sorbitol analysis by a high-performance anion-exchange chromatography-pulsed amperometric detection method. Its clinical utility as a diagnostic tool was established by measuring sorbitol in the sciatic nerves or salivary glands of diabetes mellitus-induced mice. Sorbitol was completely separated from other monosaccharides on an anion-exchange column with 100mM NaOH as eluent. The limit of detection (S/N=3) and limit of quantification (S/N=10) were 0.03 ng (3 ng/g) and 0.10 ng (10 ng/g), respectively. The linear dynamic range was 0.01-50.0 microg/g (r(2)=0.9997 and 0.9989 for sciatic nerves and salivary glands, respectively), and the mean recoveries for intra- or inter-day assays were in the range of 98.5-103.9%. This method easily identified diabetic and normal groups, making it a practical procedure for the rapid screening of diabetic neuropathy.
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