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  • Title: Chondrogenic properties of primary human chondrocytes culture in hyaluronic acid treated gelatin scaffold.
    Author: Pruksakorn D, Khamwaen N, Pothacharoen P, Arpornchayanon O, Rojanasthien S, Kongtawelert P.
    Journal: J Med Assoc Thai; 2009 Apr; 92(4):483-90. PubMed ID: 19374298.
    Abstract:
    OBJECTIVE: To study the possibility of primary human chondrocytes culture in gelatin scaffold and the effects of exogenous HA on chondrocyte differentiation and synthesis of the hyaline-like extracellular matrix. MATERIAL AND METHOD: Cartilage tissue was engineered by using primary human chondrocytes with HA-treated gelatin scaffolds and gelatin scaffolds. The chondrogenic properties were monitored for chondrocyte proliferation, adhesion, and hyaline-like extracellular matrix production in both groups. The results were compared to each other. RESULTS: Chondrocyte proliferation, adhesive activity, and new HA production were significantly increased in HA-treated gelatin scaffold (p < 0.05). Immuno histochemistry for WF6 epitope demonstrated the higher quality of hyaline-like extracellular matrix production. Moreover, the scanning electron micrograph showed a higher filling of extracellular matrix in the pore of scaffold of HA-treated gelatin scaffold than that in non-HA treated scaffold. CONCLUSION: The present study demonstrated the possible role of commercial gelatin-based scaffold in cartilage tissue engineering. It also demonstrated that exogenous HA-treated scaffold provides positive effects for chondrocytes.
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