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  • Title: Real-time microgravimetric quantification of Cryptosporidium parvum in the presence of potential interferents.
    Author: Poitras C, Fatisson J, Tufenkji N.
    Journal: Water Res; 2009 Jun; 43(10):2631-8. PubMed ID: 19375770.
    Abstract:
    The quartz crystal microbalance with dissipation monitoring (QCM-D) is used to develop a biosensor for detection of viable Cryptosporidium parvum (C. parvum) in water matrices of varying complexity. In a clean environment, a good log-log linear response is obtained for detection of C. parvum in aqueous suspensions with oocyst concentrations from 3x10(5) to 1x10(7)oocysts/mL. C. parvum detection is slightly affected by the presence of dissolved organic acids, likely due to steric stabilization and/or masking of the antibodies/antigens by adsorbed molecules. Colloidal contaminants generally have a greater influence as biosensor interferents, whereby the presence of model latex microspheres, Enterococcus faecalis, or Escherichia coli, led to decreases in biosensor response of up to 64%, 40%, and 20%, respectively.
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