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  • Title: Development and application of G1-ELISA for detection of antibodies against bovine ephemeral fever virus.
    Author: Zheng FY, Lin GZ, Qiu CQ, Zhou JZ, Cao XA, Gong XW.
    Journal: Res Vet Sci; 2009 Oct; 87(2):211-2. PubMed ID: 19376554.
    Abstract:
    The gene encoding antigenic site G(1) of bovine ephemeral fever virus (BEFV) was highly expressed in the host cell Escherichia coli. An indirect G(1)-ELISA with the recombinant protein as the coating antigen was established to detect antibodies against BEFV. The result revealed that the optimal concentration of the coated antigen was 0.5 microg/well and the dilution of serum was 1:20. It was optimal that sera with P/N >or= 2.2 were considered positive, P/N <or=2.0 negative, and between 2.0 and 2.2 ambiguous. The G(1)-ELISA method gave a sensitivity of 97.6% and a specificity of 98.6% by testing 590 field serum samples. These results suggest that the G(1)-ELISA may be a good alternative tool for seroepidemiological surveys.
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