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  • Title: Characterization of human skeletal muscle biopsy samples using shotgun proteomics.
    Author: Parker KC, Walsh RJ, Salajegheh M, Amato AA, Krastins B, Sarracino DA, Greenberg SA.
    Journal: J Proteome Res; 2009 Jul; 8(7):3265-77. PubMed ID: 19382779.
    Abstract:
    We characterized the human muscle proteome by studying muscle biopsy specimens through four different workflows, using 1 or 2D peptide separation, SDS gels, or differential solubilization. By performing MS/MS analyses of 178 4-h LC separations derived from 31 patients, we identified more than 2000 proteins, and determined how 370 very abundant proteins behave upon differential solubilization. The resulting semiquantitative database should serve as a resource for muscle biochemistry.
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