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  • Title: A high-performance liquid chromatographic analysis and preliminary pharmacokinetic characterization of 3'-hydroxypterostilbene in rats.
    Author: Takemoto JK, Davies NM.
    Journal: Biomed Chromatogr; 2009 Oct; 23(10):1086-91. PubMed ID: 19385002.
    Abstract:
    A high-performance liquid chromatographic method was developed for the analysis of 3'-hydroxypterostilbene. This method involves the use of a Luna C(18) column with ultraviolet detection at 325 nm. The mobile phase consisted of acetonitrile, water and formic acid (50:50:0.01, v/v/v) with a flow rate of 0.8 mL/min. The calibration curves were linear over the range 0.5-100.0 microg/mL. The mean extraction efficiency was between 97.40 and 111.16%. The precision of the assay was 0.196-14.39% (RSD%), and within 15% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <16% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and serum.
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