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  • Title: High-performance liquid chromatographic determination of histamine N-methyltransferase activity.
    Author: Fukuda H, Yamatodani A, Imamura I, Maeyama K, Watanabe T, Wada H.
    Journal: J Chromatogr; 1991 Jul 05; 567(2):459-64. PubMed ID: 1939478.
    Abstract:
    A method for the determination of histamine N-methyltransferase (HMT) activity by high-performance liquid chromatography based on post-column derivatization with omicron-phthalaldehyde is described. The determination involves the separation of the substrate, histamine, from its product. N tau-methylhistamine, using a weak cation exchanger, followed by on-line derivatization of these imidazoleamines with omicron-phthalaldehyde and their detection and quantitation with a fluorimetric detector. This assay method is suitable for the measurement of HMT activity during enzyme purification.
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