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  • Title: Quantitative determination of different apolipoprotein B containing lipoproteins by an enzyme linked immunosorbent assay: apo B with apo C-III and apo B with apo E.
    Author: Kandoussi A, Cachera C, Parsy D, Bard JM, Fruchart JC.
    Journal: J Immunoassay; 1991; 12(3):305-23. PubMed ID: 1939663.
    Abstract:
    A non competitive enzyme-linked immunosorbent assay (ELISA) for total apolipoprotein (apo) B, apo B with apo C-III (LpC-III:B), and apo B with apo E (LpE:B), was developed. Microtiter plates were used as solid-phase, and subdivided into three parts, coated respectively with affinity purified antibodies to apo B, to apo C-III and to apo E. After incubating the antigen with coated plates, a horseradish peroxidase-labelled antibody to apo B was added to all the plates to estimate total apo B, LpC-III:B and LpE:B.
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