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Title: Two-colour two-photon confocal microscopy with isotropic three-dimensional resolution and parallel excitation. Author: Ni J, Qiao L, Wang C, Zhao F, Cheng Y, Xu Z. Journal: J Microsc; 2009 May; 234(2):205-10. PubMed ID: 19397749. Abstract: We demonstrate a novel design of two-colour two-photon fluorescence microscope in which isotropic three-dimensional imaging resolution and high scanning speed can be achieved simultaneously. In our scheme, a three-dimensional optical lattice constructed by multi-beam interference is used for two-colour two-photon fluorescence excitation. Our simulation results show that a resolution of 113.5 nm can be achieved in both transverse and axial directions with two pump pulses at the wavelengths of 400 and 800 nm, respectively; meanwhile, imaging speed can be greatly improved compared with that of traditional two-photon scanning fluorescence microscopes.[Abstract] [Full Text] [Related] [New Search]