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  • Title: Carboxylated magnetic microbead-assisted fluoroimmunoassay for early biomarkers of acute myocardial infarction.
    Author: Wang J, Wang Q, Ren L, Wang X, Wan Z, Liu W, Li L, Zhao H, Li M, Tong D, Xu J.
    Journal: Colloids Surf B Biointerfaces; 2009 Aug 01; 72(1):112-20. PubMed ID: 19398193.
    Abstract:
    A carboxylated superparamagnetic microbead-assisted sandwich fluoroimmunoassay was successfully demonstrated for the analysis of the early protein markers, myoglobin and human heart-type fatty acid-binding protein (H-FABP), associated with acute myocardial infarction. This assay approach consisted of the preparation of superparamagnetic polymer microbeads using a dispersion polymerization, followed by grafting of capture antibodies (monoclonal anti-H-FABP 10E1 and anti-myoglobin 7C3) onto the polymer microbeads using EDC-NHS protocol, and then a sequential sandwich fluoroimmunoassay using detection antibodies (FITC-labeled anti-H-FABP 9F3 and FITC-labeled anti-myoglobin 4E2). The Fe(3)O(4) nanoparticles and carboxylated Fe(3)O(4)-polymer microbeads were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrophotometry, vibrating sample magnetometry, and X-ray diffraction. The fluoroimmunoassay images were recorded using a confocal laser-scanning microscope, and the average fluorescence intensity of the microbeads was found to correspond to the concentration of each cardiac marker, in agreement with the results obtained by a spectrofluorophotometer. The carboxylated magnetic microbead-assisted protocol could be utilized to semi-quantitatively detect both myoglobin and H-FABP.
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