These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [A simple and efficient method for establishing a mouse model of orthotopic MB49 bladder cancer].
    Author: Liang ZK, Zhang L, Hu ZM, Chen Z, Huang X, Shi XH, Tan WL, Gao JM.
    Journal: Nan Fang Yi Ke Da Xue Xue Bao; 2009 Apr; 29(4):627-30. PubMed ID: 19403380.
    Abstract:
    OBJECTIVE: To establish a simple and efficient method for establishing a mouse model of orthotopic superficial bladder cancer. METHODS: C57BL/6 mice were anesthetized with sodium pentobarbital and catheterized with modified IV catheter (24 G). The mice were intravesically pretreated with HCl and then with NaOH, and after washing the bladders with phosphate-buffered saline (PBS), 100 microl (1 x 10(7)) MB49 cells were infused and allowed to incubate in the bladder for 2 h followed intravesical mitomycin C (MMC) administration. The tumor formation rate, survival, gross hematuria, and bladder weight were determined as the outcome variables, and the pathology of the bladders was observed. RESULTS: Instillation of MB49 tumor cells resulted in a tumor formation rates of 100% in all the pretreated groups while 0% in the control group without pretreatment. MMC significantly reduced the bladder weight as compared to PBS. CONCLUSION: We have successfully established a stable, reproducible, and reliable orthotopic bladder cancer model in mice.
    [Abstract] [Full Text] [Related] [New Search]