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  • Title: Novel fluorescent probe for analysis of hydroperoxides based on boron dipyrromethane fluorophore.
    Author: Inoue N, Suzuki Y, Yokoyama K, Karube I.
    Journal: Biosci Biotechnol Biochem; 2009 May; 73(5):1215-7. PubMed ID: 19420684.
    Abstract:
    A new reagent, N-[2-(diphenylphosphino)ethyl]-4-(1,3,5,7-tetramethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-8-yl)benzamide (DPPEA-BODIPY), was designed and synthesized for analysis of hydroperoxides. DPPEA-BODIPY fluoresces at low levels in the visible range (lambda(ex)/lambda(em) = 502 nm/515 nm) and reacts with hydroperoxides to produce DPPEA-BODIPY oxide, which fluoresces at high levels. The fluorescence intensity of the reaction mixture was observed to be linearly related to the methyl linoleate hydroperoxide (MeLOOH) concentration.
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