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  • Title: Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.
    Author: Contreras R, Volckaert G, Thys F, Van de Voorde A, Fiers W.
    Journal: Nucleic Acids Res; 1977 Apr; 4(4):1001-14. PubMed ID: 194225.
    Abstract:
    The nucleotide sequence of the SV40 genome region between the Hind K fragment and the Eco RI cleavage site has been determined by a combination of three different approaches : analysis of RNA products obtained by transcription with Escherichia coli DNA dependent RNA polymerase, partial degradations with snake venom exonuclease and base-specific chemical degradation of 5'-terminal labeled restriction fragments. This nucleotide sequence shows only one open reading frame and allows the deduction of a small segment of the amino acid sequence of VP1, the major structural protein.
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