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  • Title: Chitosan-SiO2-multiwall carbon nanotubes nanocomposite: a novel matrix for the immobilization of creatine amidinohydrolase.
    Author: Tiwari A, Dhakate SR.
    Journal: Int J Biol Macromol; 2009 Jun 01; 44(5):408-12. PubMed ID: 19428474.
    Abstract:
    A matrix made up of chitosan-SiO(2)-multiwall carbon nanotubes (CHIT-SiO(2)-MWCNTs) nanocomposite was fabricated to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-SiO(2)-MWCNTs matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-SiO(2)-MWCNTs biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV) taking CHIT-SiO(2)-MWNTs as a reference. The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.58 mM and 83.16 mg/cm(2), respectively; indicating CHIT-SiO(2)-MWCNTs nanocomposite matrix has a high affinity to immobilize CAH enzyme.
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